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eBook details

• Title: Isolation and Mutagenesis of Streptokinase Producing Bacteria.
• Author : American Journal of Immunology
• Release Date : January 01, 2005
• Genre: Life Sciences,Books,Science & Nature,
• Pages : * pages
• Size : 180 KB

Description

Abstract: Of 103 [beta]-haemolytic Streptococci (with haemolytic activity) in total were isolated from different samples of blood and biomass from infected throat, 47 isolates exhibited streptokinase (SK) activity. T3, identified as a new variant of Streptococcus equisimilis, was found to be excellent SK producer in submerged fermentation and was selected for strain improvement studies. This strain was mutagenized by UV and N-methyl-N'-nitro-N-nitroso guanidine (NTG). The isolation of mutants and the SK activity of selected mutants were described. The SK yield of the best UV mutant AUV10 was 120% higher than the wild strain (T3). Also, the SK yield of the best NTG mutant NUV7 was 146% higher than the wild strain (T3). The results indicated that UV and NTG were effective mutagenic agents for strain improvement of S. equisimilis T3 for enhanced SK productivity. Key words: Streptokinase (SK), screening, isolation, mutagenesis, bacteria

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